human breast cancer cell line mcf 7 (ATCC)

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ATCC human breast cancer cell line mcf 7
Human Breast Cancer Cell Line Mcf 7, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 34824 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/breast+mcf+7/MCF7/pmc13101642-243-7-13
Average 99 stars, based on 34824 article reviews

human breast cancer cell line mcf 7 - by Bioz Stars, 2026-09

99/100 stars

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Multiple Displacement Amplification:

Article Title: Design, synthesis, anticancer activity, and mechanistic investigation of 4,5,6,7-tetrahydrobenzo[ b ]thiophene carboxamides as CDK-2 inhibitors: in vitro and in silico DFT and molecular docking study
Article Snippet: .. The examined cancer cells, hepatic HepG-2 and breast MCF-7 and MDA-MB-231, have been purchased from American Type Culture Collection (Manassas, VA). .. MTT technique was applied and the IC 50 was determined using a non-linear regression model in GraphPad Prism software (San Diego, CA).

Article Title: Oestradiol post-functionalized gold(I) bis(1,2,3-triazol-5-ylidene) complex exhibits high activity for ERα-positive breast cancer cells (MCF-7).
Article Snippet: .. The cytotoxic activity of the complexes was evaluated in the human ovarian A2780 and human dermal fibroblasts HDF (Sigma-Aldrich), breast MCF-7 and MDA-MB-231 (American Type Culture Collection) cancer cells. ..

Activity Assay:

Article Title: Design, synthesis and cytotoxic research of a novel antitumor model based on acrylamide–PABA analogs via β-tubulin inhibition
Article Snippet: .. Cytotoxic activity evaluation To measure the cytotoxic actvity of the synthesized acrylamide-PABA derivatives 4aj in breast MCF-7 (ATCC Cat. No. HTB-22), liver HepG2 (ATCC Cat No. HB-8065), and normal breast MCF-10A (ATCC Cat. No. CRL 10317) cell lines, cell viability assay was assessed using MTT assay method. ..

Synthesized:

Article Title: Synthesis of Benzopyrans and Quinolines with Nitrogenated Chain and Their Cytotoxicity Against Human Cancer Cell Lines.
Article Snippet: Human cancer cell lines were purchased from American Type Culture Collection (ATCC, Manassas, Virginia, USA). .. Synthesized benzopyrans and quinolines were assayed against the human cancer cell lines, including lung A549 (RRID: CVCL_A549; CCL-185 ATCC), melanoma A2058 (RRID: CVCL_1059; CRL_3601 ATCC), hepatoma HepG2 (RRID: CVCL_0027; HB-8065 ATCC), breast MCF-7 (RRID:CVCL_ 0031; HTB-22 ATCC), and pancreas Mia PaCa-2 (RRID: CVCL_0428; CRL-1420 ATCC) in an MTT test as 10-point curves with 1:2 dilutions starting at 40 μM in triplicate. .. Cells were seeded at 4.000 cells/well in a 384-well plate (Corning 3701) for 24 h and compounds were added with an Echo 550 (Beckman Coulter) that enables the transference of ultralow sample volumes (nanolitres), and cells were incubated for 72 h [40].

Article Title: Synthesis of Benzopyrans and Quinolines with Nitrogenated Chain and Their Cytotoxicity Against Human Cancer Cell Lines
Article Snippet: Human cancer cell lines were purchased from American Type Culture Collection (ATCC, Manassas, Virginia, USA). .. Synthesized benzopyrans and quinolines were assayed against the human cancer cell lines, including lung A549 (RRID:CVCL_A549; CCL‐185 ATCC), melanoma A2058 (RRID:CVCL_1059; CRL_3601 ATCC), hepatoma HepG2 (RRID:CVCL_0027; HB‐8065 ATCC), breast MCF‐7 (RRID:CVCL_0031; HTB‐22 ATCC), and pancreas Mia PaCa‐2 (RRID:CVCL_0428; CRL‐1420 ATCC) in an MTT test as 10‐point curves with 1:2 dilutions starting at 40 μM in triplicate. .. Cells were seeded at 4.000 cells/well in a 384‐well plate (Corning 3701) for 24 h and compounds were added with an Echo 550 (Beckman Coulter) that enables the transference of ultralow sample volumes (nanolitres), and cells were incubated for 72 h [ ].

MTT Assay:

Cell Culture:

Article Title: Proteomic exploration of the recently Re-classified forest cobra Naja species and the potential cytotoxic activity in cancer cell lines.
Article Snippet: .. In this study, the pancreatic MIA PaCa-2 (ATCC: CRL-1420), cervical HeLa (ATCC: CCL-2), breast MCF-7 (ATCC: HTB-22), uterus ME-180 (ATCC: HTB-33), melanoma A375 (ATCC: CRL-1619), prostate PC-3 (ATCC: CRL-1435) cancer cell lines, and human fetal lung MRC-5 (ATCC: CCL-171) normal cell lines were cultured in Dulbecco’s Modified Eagles Medium (DMEM; Life Technologies Corporation Limited, UK) supplemented with 1 % (v/v) Amphotericin B (Life Technologies Corporation Limited, UK), 1 % (v/v) Pen-Strep antibiotic (Penicillin Streptomycin) (Life Technologies Corporation Limited, USA), and 10 % (v/v) Fetal Bovine Serum (FBS; Life Technologies Corporation Limited, UK). .. The cells were grown in 75 cm2 culture flasks and cultured in a HERAcell 150i incubator (Thermo Fisher Scientific, South Africa) at 37 ◦C in a 95 % humidified atmosphere at 5 % CO2, until reaching a state of confluence.

Modification:

other:

Article Title: Glyco-Conjugation of Thiosemicarbazone Copper(II) Complexes Enhances Selectivity toward Cancer Cells Overexpressing GLUT1
Article Snippet: Human colon (HCT-15), breast (MCF-7) and pancreatic (PSN-1) carcinoma cell lines were obtained by American Type Culture Collection (ATCC, Rockville, MD, USA).

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